Proton Nuclear Magnetic Resonance of Intact Friend Leukemia Cells: Phosphorylcholine Increase During Differentiation
- 18 June 1982
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 216 (4552), 1325-1327
- https://doi.org/10.1126/science.7079765
Abstract
Proton nuclear magnetic resonance of intact Friend leukemia cells was used to analyze their erythroid-like differentiation. The technique, which requires only 10(3) to 10(9) cells and approximately 2 minutes for acquisition of each spectrum, demonstrated the occurrence of many signal changes during differentiation. With cell extracts, 64 signals were assigned to 12 amino acids and 19 other intermediary metabolites, and a dramatic signal change was attributed to a fourfold increase in cytoplasmic phosphorylcholines.This publication has 12 references indexed in Scilit:
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