Evidence for Different Human Tracheobronchial Mucin Peptides Deduced from Nucleotide cDNA Sequences
- 1 August 1991
- journal article
- Published by American Thoracic Society in American Journal of Respiratory Cell and Molecular Biology
- Vol. 5 (2), 178-185
- https://doi.org/10.1165/ajrcmb/5.2.178
Abstract
Highly glycosylated regions or glycopeptides were obtained by proteolysis of human tracheobronchial mucins. They were chemically deglycosylated and the resulting products were used to raise a rabbit antiserum. This antiserum specifically recognized the superanuclear region of respiratory and colonic goblet cells as areas around and below the nucleus of mucin-secreting cells in tracheobronchial mucous glands. A lambda gt11 cDNA library constructed from human tracheobronchial mucosa was screened with this antiserum. Ten positive clones were obtained from screening half of the library (about 10(6) recombinants). The antibodies were purified by absorption to each positive clone; some purified antibodies were specific for goblet cells and others recognized both goblet and mucous cells, indicating that there is differential cellular expression of mucin peptides. The total or partial amino acid sequences deduced from these cDNA clones could be classified into three groups. The first group contained repetitive sequences of eight amino acid residues, almost perfectly identical, and in different arrangements. The second type exhibited homology at their amino and carboxy-terminal ends. The last group had no distinctive feature except for a high content of hydroxy amino acids typical of mucins. Five different clones could correspond to the carboxy-terminal end of tracheobronchial apomucins. These results indicate that human tracheobronchial apomucins consist of a family of different proteins.Keywords
This publication has 23 references indexed in Scilit:
- Multiple apomucin translation products from human respiratory mucosa mRNAEuropean Journal of Biochemistry, 1991
- Molecular cloning and chromosomal localization of a novel human tracheo-bronchial mucin cDNA containing tandemly repeated sequences of 48 base pairsBiochemical and Biophysical Research Communications, 1991
- Molecular cloning of cDNAs derived from a novel human intestinal mucin geneBiochemical and Biophysical Research Communications, 1990
- Use of an antiserum against deglycosylated human mucins for cellular localization of their peptide precursors: antigenic similarities between bronchial and intestinal mucins.Journal of Histochemistry & Cytochemistry, 1989
- The complexity of mucinsBiochimie, 1988
- Flexibility and length of human bronchial mucin studied using low‐shear viscometry, birefringence relaxation analysis, and electron microscopyBiopolymers, 1985
- Resolution of the major components of human lung mucosal gel and their capabilities for reaggregation and gel formationBiochemistry, 1979
- Empirical Predictions of Protein ConformationAnnual Review of Biochemistry, 1978
- Limulus polyphemus lectin sites in human bronchial mucosa.Journal of Histochemistry & Cytochemistry, 1977
- Purification et études des mucines de kystes bronchogéniquesBiochimica et Biophysica Acta (BBA) - General Subjects, 1973