Location of a High-sulphur Protein in Developing Wool Follicles Using Peroxidase-Labelled Antibody

Abstract

Antiserum to a high-S protein (SCMK-B2) from wool was purified to obtain the immunoglobin G fraction, and this was labeled with horseradish peroxidase. The labeled antibody was then applied to sections of sheep skin embedded in glycol methacrylate in order to locate the distribution of high-S protein within the lower hair shaft. Regions containing the highest concentration of high-S protein were the cuticle of the hair shaft and the keratogenous zone of the cortex.