The Molecular Basis of Codominant Expression of the Histocompatibility-2 Genetic Region
- 1 June 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (6), 1394-1397
- https://doi.org/10.1073/pnas.69.6.1394
Abstract
H-2 alloantigens in an intact, undegraded form can be solubilized by the nonionic detergent NP-40 and isolated by indirect immunoprecipitation and electrophoresis in sodium dodecyl sulfate on polyacrylamide gels. With the use of an immunological precipitation method, the question about the number of cellular H-2 gene products in heterozygous cells has been partially resolved. At least four separable gene products were detected in cells heterozygous at the H-2 genetic region-one for each of the H-2D genes of the two parental haplotypes, and one for each of the H-2K genes of the two parental haplotypes. This is in contrast to the two H-2 gene products reported previously for cells homozygous at the H-2 region. The findings establish that the two parental H-2 haplotypes on homologous chromosomes in heterozygous cells are ultimately expressed as different glycoprotein molecules.Keywords
This publication has 12 references indexed in Scilit:
- Isolation of H-2 Alloantigens Solubilized by the Detergent NP-40The Journal of Immunology, 1971
- Evidence that H-2 private specificities can be arranged in two mutually exclusive systems possibly homologous with two subsystems of HL-A.1971
- Genetic organization and evolution of the mouse H-2 region: a duplication model.1971
- HEMAGGLUTINATION AND CYTOTOXIC STUDIES OF H-2Transplantation, 1971
- The H-2 Model for the Major Histocompatibility SystemsImmunological Reviews, 1971
- Transplantation AntigensAdvances in Immunology, 1970
- Murin histocompatibility-2 (H-2) alloantigens. Purification and some chemical properties of soluble products from H-2b and H-2d genotypes released by papain digestion of membrane fractionsBiochemistry, 1969
- Genetic control of the antibody response in inbred mice. Transfer of response by spleen cells and linkage to the major histocompatibility (H-2) locus.1968
- Autonomy of H-2 Genes in Individual ImmunocytesNature, 1967
- The Ss system of the mouse--a quantitative serum protein difference genetically controlled by the H-2 region.1965