A STUDY OF FIBRINOGEN PRODUCTION BY HUMAN LIVER SLICES IN VITRO BY AN IMMUNOPRECIPITIN METHOD

Open Access
Publisher Website
Google Scholar
Abstract
After incubation of human liver slices of 7 individuals in a heparinized Krebs-Ringer-bicarbonate medium, a marked increase was noted of a protein precipitable by specific rabbit antibodies prepared against human fibrinogen. A net increase of radioactivity in the antigen-antibody-precipitate occurred when radioactive L-Leucine-C14 was added to the incubation medium. The production of an average of 0.19 mg of fibrinogen/g liver/4 hours was in the order of magnitude of the fibrinogen production calculated from in vivo turnover studies.