Role of a serum phospholipase A1in the phosphatidylserine‐induced T cell inhibition
- 18 January 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 316 (1), 1-4
- https://doi.org/10.1016/0014-5793(93)81724-e
Abstract
We have previously shown that unsaturated phosphatidylserines inhibit mitogen-induced T cell activation. We now report that the inhibitory action requires a protein present in bovine and human serum. Partial purification and phospholipase assay show that this protein has phospholipase A activity on phosphatidylserine but not phosphatidylethanolamine, phosphatidylcholine and phosphatidylinositol. In short incubations (1–3 h) 2-acyl lysophosphatidylserine is produced but in longer incubations the cis-unsaturated fatty acid is also released. Experiments on peripheral blood mononuclear cells indicate that the unsaturated fatty acid becomes the main responsible for the PS-induced inhibition and that 2-acyl lysophosphatidylserine enhances the inhibitory effect of fatty acid.Keywords
This publication has 17 references indexed in Scilit:
- Inhibition of DNA synthesis in peripheral blood mononuclear cells treated with phosphatidylserines containing unsaturated acyl chainsImmunopharmacology, 1992
- Inhibition of phospholipase A2 by heparinBiochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1990
- Signalling mechanism in the lysophosphatidylserine-induced activation of mouse mast cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1990
- Lysophosphatidylserine-dependent interaction between rat leukocytes and mast cellsBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1987
- Immunoregulation by low density lipoproteins in man. Inhibition of mitogen-induced T lymphocyte proliferation by interference with transferrin metabolism.JCI Insight, 1984
- Isolation and characterization of human plasma lipid transfer proteins.Arteriosclerosis: An Official Journal of the American Heart Association, Inc., 1984
- Intracellular phospholipases ABiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1980
- The formation of phosphatidylinositol by acylation of 2-acyl-sn-glycero-3-phosphorylinositol in rat liver microsomesLipids, 1979
- Phospholipid hydrolysis by phospholipases A1 and A2 in plasma membranes and microsomes of rat liverBiochimica et Biophysica Acta (BBA) - Biomembranes, 1973