The Nucleotide Sequence of an Equine Herpesvirus 4 Gene Homologue of the Herpes Simplex Virus 1 Glycoprotein H Gene
- 1 August 1990
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 71 (8), 1793-1800
- https://doi.org/10.1099/0022-1317-71-8-1793
Abstract
The equine herpesvirus 4 (EHV-4) gene glycoprotein H (gH) gene homologue was localized by virtue of the conserved genomic position of this gene throughout members of the herpesvirus family. The gene maps immediately downstream of the the thymidine kinase gene at approximately 0.49 to 0.51 map units within genomic fragment BamHI C. The EHV-4 gH primary translation product is predicted to be a polypeptide of Mr 94 100, 855 amino acid long, which possesses features characteristic of a membrane glycoprotein, namely an N-terminal signal sequence, a large hydrophilic domain containing 11 putative N-linked glycosylation sites, a C-terminal transmembrane domain, and a charged cytoplasmic tail. Comparision to other herpesvirus glycoproteins revealed identifies of 85%, 26% and 32% with the gH counterparts of the alphaherpesviruses EHV-1, herpes simplex virus 1 and varicella-zoster, respectively, and of 17% and 18% with those of human cytomegalovirus, herpesvirus saimiri and Epstein-Barr virus. The EHV-4 gH exhibites features previously reported to be conserved throughout the gH polypeptides of herpesviruses of all three subgroups. A region of direct repeat elements and a possible origin of DNA replication are located immediately downstream of the gH gene.This publication has 33 references indexed in Scilit:
- The properties and sequence of glycoprotein H of herpes simplex virus type 1Virology, 1986
- Cloning and characterization of oriL2, a large palindromic DNA replication origin of herpes simplex virus type 2Journal of Virology, 1986
- Neutralization epitope of varicella zoster virus on native viral glycoprotein gp118 (VZV glycoprotein gplll)Virology, 1986
- DNA sequence and expression of the B95-8 Epstein—Barr virus genomeNature, 1984
- Characterization of the TRs/IRS origin of DNA replication of herpes simplex virus Type 1Virology, 1983
- Prediction of protein antigenic determinants from amino acid sequences.Proceedings of the National Academy of Sciences, 1981
- The involvement of herpes simplex virus type 1 glycoproteins in cell-mediated immunity.The Journal of Immunology, 1981
- Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteinsJournal of Molecular Biology, 1978
- DNA sequencing with chain-terminating inhibitorsProceedings of the National Academy of Sciences, 1977
- 3′ Non-coding region sequences in eukaryotic messenger RNANature, 1976