A HISTOCHEMICAL ANALYSIS OF DEHYDROGENASE VARIETY IN THE DIFFERENT TYPES OF MUSCULAR TISSUES

Abstract

Histochemically, we examined several types of dehydrogenases in the myoblasts from "in vitro" cultures of the chick embryos, in the gastroenemius of frog, in the pigeon pectoralis, in the heart of several animal species and in the myometrium of the rabbit in different functional states (non-gestatory, gestatory and postpartum In the myoblasts were active: succinic, isocitric, malic dehydrogenases, di- and triphosphopyridine nucleotide diaphorases, glutamic, α-glycerophosphate and lactic dehydrogenases and lactic acid, the two dehydrogenases of the pentose cycle and β-hydroxybutyric dehydrogenase. The cardiac muscular tissues and red striated muscle show high and very high reactions for the enzymes of the Krebs cycle and the diaphorases, and negative reactions for the rest of the studied enzymes. The white striated muscular tissue presents all the reactions, except those of the enzymes of the hexose shunt. This type of tissue is marked by a high enzyme activity of the anaerobic glycolysis. The gestatory and non-gestatory myometrium shows all the enzymatic reactions except lactic dehydrogenase. In the myometrium of the postpartum period one notes an enzyme decrease of the Krebs cycle, of the pentose cycle and of the lipidic metabolism and an intensification of the anaerobic glycolysis and the glucose dehydrogenase. Alcohol dehydrogenase was negative in all tissues studied with the exception of one weak reaction in the myometrium during the first postpartum hours.