The γ subunit in chloroplast F1‐ATPase can rotate in a unidirectional and counter‐clockwise manner
Open Access
- 7 December 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 463 (1-2), 35-38
- https://doi.org/10.1016/s0014-5793(99)01602-6
Abstract
Rotation of the γ subunit in chloroplast F1‐ATPase (CF1) was investigated by using a single molecule observation technique, which is developed by Noji et al. to observe the rotation of a central γ subunit portion in the α3β3γ sub‐complex of F1‐ATPase from thermophilic Bacillus PS3 (TF1) during ATP hydrolysis [Noji, H. et al. (1997) Nature 386, 299–302]. We used two cysteines of the γ subunit (Cys‐199 and Cys‐205) of CF1‐ATPase, which are involved in the regulation of this enzyme, to fix the fluorochrome‐labeled actin filament. Then we successfully observed a unidirectional, counter‐clockwise rotation of the actin filament with the fluorescent microscope indicating the rotation of the γ subunit in CF1‐ATPase. We conclude that the rotation of the γ subunit in the F1‐motor is a ubiquitous phenomenon in all F1‐ATPases in prokaryotes as well as in eukaryotes.This publication has 22 references indexed in Scilit:
- Rotation of Escherichia coli F1-ATPaseBiochemical and Biophysical Research Communications, 1999
- One of the non-exchangeable nucleotides of the mitochondrial F1-ATPase is bound at a β-subunit: evidence for a non-rotatory two-site catalytic mechanismBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1999
- New results about structure, function and regulation of the chloroplast ATP synthase (CF0CF1)Physiologia Plantarum, 1999
- The 20 C-terminal Amino Acid Residues of the Chloroplast ATP Synthase γ Subunit Are Not Essential for ActivityPublished by Elsevier ,1999
- Direct Observation of the Rotation of ε Subunit in F1-ATPaseJournal of Biological Chemistry, 1998
- F1-ATPase Is a Highly Efficient Molecular Motor that Rotates with Discrete 120° StepsCell, 1998
- The Formation or the Reduction of a Disulfide Bridge on the γ Subunit of Chloroplast ATP Synthase Affects the Inhibitory Effect of the ε SubunitPublished by Elsevier ,1998
- The Regulatory Functions of the γ and ɛ Subunits from Chloroplast CF1 are Transferred to the Core Complex, α3β3, from Thermophilic Bacterial F1European Journal of Biochemistry, 1997
- Direct observation of the rotation of F1-ATPaseNature, 1997
- Binding stoichiometry and structural mapping of the .epsilon. polypeptide of chloroplast coupling factor 1Biochemistry, 1985