DNA‐damaging activity of biotic and xenobiotic aldehydes in chinese hamster ovary cells

Abstract

Alkaline elution was employed to study DNA damage in CHO‐Kl cells treated with a series of biotic and xenobiotic aldehydes. DNA cross‐linking was measured in terms of the reduction in the effect of methyl methanesulphonate on the kinetics of DNA elution and was observed in cells treated with formaldehyde, acetaldehyde, methylglyoxal and malonaldehyde. Propionaldehyde, valeraldehyde, hexanal and 4‐hydroxynonenal produced DNA single‐strand breaks, or lesions which were converted to breaks in alkali. Both types of DNA damage occurred in cells exposed to malealdehyde. These findings support the hypothesis of a carcinogenic effect of the aldehydic products (malonaldehyde, methylglyoxal, propionaldehyde, hexanal, 4‐hydroxynonenal) released in biomembranes during lipid peroxidation.