Purification of Rabbit Skeletal Muscle Troponin C.
- 1 January 1988
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 42b (4), 211-215
- https://doi.org/10.3891/acta.chem.scand.42b-0211
Abstract
Troponin C binds to phenyl-Sepharose in the presence of Ca2+ and can be eluted with EDTA. This property was used as an essential step in the purification of this protein from rabbit skeletal muscle. Troponin C was extracted with 6M urea from extensively washed ground muscle. The protein was bound to and eluted from DEAE-Sephadex, fractionated by size on Sephadex G75, and in a final step purified from UV-absorbing non-protein impurities on phenyl-Sepharose. The total yield of electrophoretically pure protein was 60 mg per 100 g of muscle, which is considerably higher than that previously obtained.This publication has 15 references indexed in Scilit:
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