Age-Associated Decline in the in Vitro Development of Cytotoxic Lymphocytes in NZB Mice
- 1 October 1976
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 117 (4), 1093-1098
- https://doi.org/10.4049/jimmunol.117.4.1093
Abstract
The development of cytotoxic lymphocytes (CL) in in vitro mixed lymphocyte culture (MLC) was investigated in young (14 weeks), middle (40 weeks), and aged (80 weeks) NZB mice. Cytotoxic activity against H-2b alloantigens was measured by using the 51Cr release assay. The antigen dose to elicit the optimum development of CL in vitro was the same for all ages of NZB mice, but the level of the development of CL was consistently low and could be delayed by up to 24 hr in aged mice. This decline in the development of CL was not related to the increase of the frequency of autoantibody against red blood cells nor to the decrease of the frequency of θ-positive cells in aged NZB mice. Aged (83 weeks) DBA/2 mice showed a similar decline in the development of CL. This decline of T cell function in aged NZB mice might be related to a physiologic aging process rather than to autoimmune disease.This publication has 5 references indexed in Scilit:
- CELL-MEDIATED IMMUNE-RESPONSE INVITRO .1. DEVELOPMENT OF SUPPRESSOR CELLS AND CYTOTOXIC LYMPHOCYTES IN MIXED LYMPHOCYTE-CULTURES1976
- Deficient Immunologic Functions of NZB MiceExperimental Biology and Medicine, 1968
- IMMUNIZATION OF DISSOCIATED SPLEEN CELL CULTURES FROM NORMAL MICEThe Journal of Experimental Medicine, 1967
- LOCALIZATION OF MOUSE ISOANTIGENS ON CELL SURFACE AS REVEALED BY IMMUNOFLUORESCENCE1967
- Age-Dependent Tumor-Host Barrier and Effect of Carcinogen-Induced Immunodepression on Rejection of Isografted Methylcholanthrene-Induced Sarcoma Cells2JNCI Journal of the National Cancer Institute, 1966