Evaluation of the Errors Involved in Estimating Bacterial Numbers by the Plating Method

Abstract
The dilution error and the distribution error are the chief sources of variation accounting for the total error. An expression is derived for evaluating the dilution error, deviations in pipettes and dilution blanks being known. A convenient table of dilution errors, as percentage standard deviations, is given, covering the range of dilutions ordinarily employed, for all combinations of dilution blanks and for various combinations of deviations in pipettes and blanks. The usual method for estimating the distribution error is indicated, and a formula given for calculating the total error from the distribution error and the dilution error.