Architecture of Succinate Dehydrogenase and Reactive Oxygen Species Generation
Top Cited Papers
- 31 January 2003
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 299 (5607), 700-704
- https://doi.org/10.1126/science.1079605
Abstract
The structure of Escherichia colisuccinate dehydrogenase (SQR), analogous to the mitochondrial respiratory complex II, has been determined, revealing the electron transport pathway from the electron donor, succinate, to the terminal electron acceptor, ubiquinone. It was found that the SQR redox centers are arranged in a manner that aids the prevention of reactive oxygen species (ROS) formation at the flavin adenine dinucleotide. This is likely to be the main reason SQR is expressed during aerobic respiration rather than the related enzyme fumarate reductase, which produces high levels of ROS. Furthermore, symptoms of genetic disorders associated with mitochondrial SQR mutations may be a result of ROS formation resulting from impaired electron transport in the enzyme.Keywords
This publication has 30 references indexed in Scilit:
- Molecular Basis of Proton Motive Force Generation: Structure of Formate Dehydrogenase-NScience, 2002
- Inborn errors of complex II – Unusual human mitochondrial diseasesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2001
- A Defect in the Cytochrome b Large Subunit in Complex II Causes Both Superoxide Anion Overproduction and Abnormal Energy Metabolism in Caenorhabditis elegansJournal of Biological Chemistry, 2001
- Retention of Heme in Axial Ligand Mutants of Succinate-Ubiquinone Oxidoreductase (Complex II) from Escherichia coliPublished by Elsevier ,2001
- A motif for quinone binding sites in respiratory and photosynthetic systemsJournal of Molecular Biology, 2000
- Respiration Without O 2Science, 1999
- Inhibitor Probes of the Quinone Binding Sites of Mammalian Complex II and Escherichia coli Fumarate ReductaseJournal of Biological Chemistry, 1996
- Mutation of a nuclear succinate dehydrogenase gene results in mitochondrial respiratory chain deficiencyNature Genetics, 1995
- Evidence for non‐cysteinyl coordination of the [2Fe‐2S] cluster in Escherichia coli succinate dehydrogenaseFEBS Letters, 1992
- Improved methods for building protein models in electron density maps and the location of errors in these modelsActa Crystallographica Section A Foundations of Crystallography, 1991