Engineered Two‐Helix Small Proteins for Molecular Recognition
- 14 May 2009
- journal article
- research article
- Published by Wiley in ChemBioChem
- Vol. 10 (8), 1293-1296
- https://doi.org/10.1002/cbic.200900062
Abstract
Less is more: By starting with a high‐affinity HER2‐binding 3‐helix affibody molecule, we successfully developed 2‐helix small protein binders with 5 nM affinities by using a combination of several different strategies. Our efforts clearly suggest that 2‐helix small proteins against important tumor targets can be obtained by rational protein design and engineering.Keywords
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