Histone H4 hyperacetylation and rapid turnover of its acetyl groups in transcriptionally inactive rooster testis spermatids
Open Access
- 1 January 1982
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 10 (24), 8049-8059
- https://doi.org/10.1093/nar/10.24.8049
Abstract
In order to study the relationship between acetylation of histones, chromation structure and gene activity, the distribution and turnover of acetyl groups among nucleosomal core histones and the extent of histone H4 acetylation were examined in rooster testis cell nuclei at different stages of spermatogenesis. Histone H4 was the predominant acetylated histone in mature testes. Hyperacetylation of H4 and rapid turnover of its acetyl groups are not univocally correlated with transcriptional activity since they were detected in both genetically active testicular cells and genetically inactive elongated spermatids. During the transition from nucleohistone to nucleprotamine in elongated spermatids the chromatin undergoes dramatic structural changes with exposition of binding sites on DNA (1). Hyperacetylation of H4 and rapid turnover of its acetyl groups could be correlated with the particular conformation of chromatin in elongated spermatids and might represent a necessary condition for binding of chromosomal proteins to DNA.Keywords
This publication has 12 references indexed in Scilit:
- Acetylation of histones in nucleosomesMolecular and Cellular Biochemistry, 1982
- Acetylation of histone H4 in chicken erythrocyte and cuttle‐fish testis chromatinFEBS Letters, 1981
- Acetylation of histone H4 and its role in chromatin structure and functionNature, 1980
- Modification of histones immediately following synthesisArchives of Biochemistry and Biophysics, 1979
- Studies on sex-organ development. Changes in chromatin structure during spermatogenesis in maturing rooster testis as demonstrated by the initiation pattern of ribonucleic acid synthesis in vitroBiochemical Journal, 1978
- Resolution of histones by polyacrylamide gel electrophoresis in presence of nonionic detergents.1978
- Studies on sex-organ development. Changes in nuclear and chromatin composition and genomic activity during spermatogenesis in the maturing rooster testisBiochemical Journal, 1977
- Quantitative Film Detection of 3H and 14C in Polyacrylamide Gels by FluorographyEuropean Journal of Biochemistry, 1975
- Acetylation of histones of rat testisArchives of Biochemistry and Biophysics, 1975
- Histone deacetylase from calf thymusBiochimica et Biophysica Acta (BBA) - Enzymology, 1970