Inactivation of an aldehyde/alcohol dehydrogenase gene fromClostridium acetobutylicum ATCC 824
- 1 March 1996
- journal article
- Published by Springer Nature in Applied Biochemistry and Biotechnology
- Vol. 57-58 (1), 213-221
- https://doi.org/10.1007/bf02941702
Abstract
A nonreplicative plasmid containing an internalaad gene fragment has been integrated into the chromosome ofClostridium acetobutylicum ATCC 824. Transformation was accomplished by electroporation with relatively high concentrations of methylated plasmid DNA. Southern hybridization experiments revealed that integration occurred by single crossover homologous recombination inactivating theaad gene. Integrants were relatively stable after 25 generations. Inactivation of theaad gene drastically reduced solvent production. This result suggests that aldehyde/alcohol dehydrogenase (AAD) plays a important role in butanol production.Keywords
This publication has 22 references indexed in Scilit:
- Targeted integration of genes into the Clostridium acetobutylicum chromosomeMicrobiology, 1994
- Construction ofEscherichia coli-Clostridium acetobutylicum shuttle vectors and transformation ofClostridium acetobutylicum strainsBiotechnology Letters, 1992
- Expression of Cloned Homologous Fermentative Genes in Clostridium Acetobutylicum ATCC 824Nature Biotechnology, 1992
- Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicumJournal of General Microbiology, 1990
- Recent advances in the genetics of the clostridiaFEMS Microbiology Letters, 1989
- Plasmid replication stimulates DNA recombination in Bacillus subtilisJournal of Molecular Biology, 1987
- The effect of pH on nitrogen supply, cell lysis, and solvent production in fermentations of Clostridium acetobutylicumBiotechnology & Bioengineering, 1985
- Use of Integrational Plasmid Vectors to Demonstrate the Polycistronic Nature of a Transcriptional Unit (spoIIA) Required for Sporulation of Bacillus subtilisMicrobiology, 1984
- Gene Amplification in Bacillus subtilisMicrobiology, 1984
- Detection of specific sequences among DNA fragments separated by gel electrophoresisJournal of Molecular Biology, 1975