Cap Z(36/32), a barbed end actin-capping protein, is a component of the Z-line of skeletal muscle.
Open Access
- 1 July 1987
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 105 (1), 371-379
- https://doi.org/10.1083/jcb.105.1.371
Abstract
Various biological activities have been attributed to actin-capping proteins based on their in vitro effects on actin filaments. However, there is little direct evidence for their in vivo activities. In this paper, we show that Cap Z(36/32), a barbed end, actin-capping protein isolated from muscle (Casella, J. F., D. J. Maack, and S. Lin, 1986, J. Biol. Chem., 261:10915-10921) is localized to the barbed ends of actin filaments by electron microscopy and to the Z-line of chicken skeletal muscle by indirect immunofluorescence and electron microscopy. Since actin filaments associate with the Z-line at their barbed ends, these findings suggest that Cap Z(36/32) may play a role in regulating length, orienting, or attaching actin filaments to Z-discs.This publication has 38 references indexed in Scilit:
- A re-evaluation of cytoplasmic gelsolin localization.The Journal of cell biology, 1986
- Fine structure of wide and narrow vertebrate muscle Z-linesJournal of Molecular Biology, 1985
- Muscle gelsolin: isolation from heart tissue and characterization as an integral myofibrillar proteinFEBS Letters, 1984
- Unphosphorylated gelsolin is localized in regions of cell-substratum contact or attachment in Rous sarcoma virus-transformed rat cells.The Journal of cell biology, 1984
- The association of desmin with the developing myofibrils of cultured embryonic rat heart myocytesDevelopmental Biology, 1982
- Identification of gelsolin, a Ca2+-dependent regulatory protein of actin gel-sol transformation, and its intracellular distribution in a variety of cells and tissues.The Journal of cell biology, 1981
- The existence of an insoluble Z disc scaffold in chicken skeletal muscleCell, 1978
- M-protein from chicken pectoralis muscle: Isolation and characterizationJournal of Molecular Biology, 1977
- Studies on purified α-actinin: II. Electron microscopic studies on the competitive binding of α-actinin and tropomyosin to Z-line extracted myofibrilsJournal of Molecular Biology, 1972
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970