A Polygalacturonate Lyase Produced by Lachnospira multiparus Isolated from the Bovine Rumen
- 1 March 1980
- journal article
- research article
- Published by Microbiology Society in Microbiology
- Vol. 117 (1), 193-199
- https://doi.org/10.1099/00221287-117-1-193
Abstract
A poly(1,4-.alpha.-D-galacturonide) lyase (EC 4.2.2.2) from the culture fluid of L. multiparus was purified about 20-fold. The optimum pH and temperature for enzyme activity were 8.0 and 40.degree. C. The enzyme required Ca2+ and was inhibited by EDTA; it preferred polygalacturonate as substrate, cleaving 1,4-.alpha.-glycosidic linkages randomly to form unsaturated galacturonates, mainly the unsaturated digalacturonate. Some properties of the crude and purified enzyme preparations are described. An exopolygalacturonase is also produced by this organism.Keywords
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