Thymine glycol and thymidine glycol in human and rat urine: a possible assay for oxidative DNA damage.
- 1 September 1984
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 81 (18), 5633-5637
- https://doi.org/10.1073/pnas.81.18.5633
Abstract
Thymine glycol is a DNA damage product ionizing radiation and other oxidative mutagens. To find a noninvasive assay for oxidative DNA damage in individuals, a HPLC [high performance liquid chromatography] assay was developed for free thymine glycol and thymidine glycol in urine. Humans excrete .apprx. 32 nmol of the 2 glycols/day. Rats, which have a higher specific metabolic rate and shorter life span, excrete .apprx. 15 times more thymine glycol plus thymidine glycol/kg of body wt than do humans. Thymine glycol and thymidine glycol are likely to be derived from repair of oxidized DNA, rather than from alternative sources such as the diet or bacterial flora. This noninvasive assay of DNA oxidation products may allow the direct testing of current theories which relate oxidative metabolism to the processes of aging and cancer in man.Keywords
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