Evidence for the presence of Thy‐1 on cultured thymic epithelial cells of mice and rats
- 1 October 1978
- journal article
- research article
- Published by Wiley in European Journal of Immunology
- Vol. 8 (10), 728-730
- https://doi.org/10.1002/eji.1830081011
Abstract
Thymic tissue of C57BL/6 mice and DA rats was cultured. After 6–8 weeks, cultures were analyzed for their capacity to absorb anti-Thy-1 serum, and for the expression of Thy-1 on the surface of different cell types by means of the indirect peroxidase labeling method. Three main cell types were identified: epithelium-like cells, fibrocyte-like cells and macrophages, but no lymphocytes were found. The presence of Thy-1 on cultured nonlymphocytic thymus-derived cells was demonstrated by their ability to absorb the cytotoxic activity of the appropriate anti-Thy-1 sera. Electron microscopical analyses of labeling experiments revealed that Thy-1 was predominantly expressed on epithelium-like cells with preference for their cell protrusions. The possible role of Thy-1 expression, both on thymocytes and thymus epithelium for cellular interaction, is discussed.This publication has 21 references indexed in Scilit:
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