Ras p21 as a Potential Mediator of Insulin Action in Xenopus Oocytes
- 15 May 1987
- journal article
- research article
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 236 (4803), 840-843
- https://doi.org/10.1126/science.3554510
Abstract
The oncogene protein product (p21) of the ras gene has been implicated in mediating the effects of a variety of growth factors and hormones. Microinjection of monoclonal antibody 6B7, which is directed against a synthetic peptide corresponding to a highly conserved region of p21 (amino acids 29 to 44) required for p21 function, specifically inhibited Xenopus oocyte maturation induced by incubation with insulin. The inhibition was dose-dependent and specific since (i) the same antibody had no effect on progesterone-induced maturation, (ii) immunoprecipitation and Western blotting indicated that the antibody recognized a single protein of molecular weight 21,000 in oocyte extracts, and (iii) inhibition was not observed with identical concentrations of normal immunoglobulin. Thus, p21 appears to be involved in mediating insulin-induced maturation of Xenopus oocytes. Furthermore, the mechanism may involve phosphorylation of p21, as p21 was found to be a substrate of the insulin receptor kinase.This publication has 45 references indexed in Scilit:
- Purification and characterization of the receptor for insulin-like growth factor IBiochemistry, 1986
- Requirement for c-ras proteins during viral oncogene transformationNature, 1986
- Action of insulin modulated by pertussis toxin in rat adipocytesCanadian Journal of Physiology and Pharmacology, 1985
- Differential activation of yeast adenylate cyclase by wild type and mutant RAS proteinsCell, 1985
- Insulin-induced formation of ruffling membranes of KB cells and its correlation with enhancement of amino acid transport.The Journal of cell biology, 1984
- Adenylate cyclase in Xenopus laevis oocytes: Characterization of the progesterone-sensitive, membrane-bound formMolecular and Cellular Endocrinology, 1982
- Differential inhibition by progesterone of the adenylate cyclase of oocytes and follicle cells of Xenopus laevisFEBS Letters, 1982
- A study of the induction of cell division in amphibian oocytes by insulinDevelopmental Biology, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Progesterone-induced meiosis in xenopus laevis oocytes: a role for cAMP at the “maturation-promoting factor” levelCell, 1978