Aspartic acid‐121 functions at the active site of bovine pancreatic ribonuclease
- 11 June 1984
- journal article
- Published by Wiley in FEBS Letters
- Vol. 171 (2), 253-256
- https://doi.org/10.1016/0014-5793(84)80498-6
Abstract
The fully active semisynthetic enzyme formed by the non-covalent interaction of residues 1–118 of bovine pancreatic ribonuclease and a synthetic tetradecapeptide containing residues 111–124 of the enzyme has allowed a direct test of the role of aspartic acid-121 in the functioning of the molecule. Replacement of this residue by asparagine results in a derivative that is 4.5% active against cytidine 2',3'-cyclic phosphate at pH 7.0 under standard assay conditions. Further studies with the same substrate at pH 5.8 reveal that the reduced activity results entirely from a diminished catalytic efficiency and not from a decreased affinity for substrateKeywords
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