Preparation of 32P-labelled adenosine 5′-phosphate, inosine 5′-phosphate and ribose 5-phosphate
- 1 November 1954
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 58 (3), 503-506
- https://doi.org/10.1042/bj0580503
Abstract
A method is described for the preparation of P32-labeled adenosine 5[image]-phosphate in an extract of brewer''s yeast. The /product is quantitatively separated and is chromatographically homogeneous. It has an activity of approximately 0.2 mC/mg. After deamination of this adenosine 5[image]-phosphate with nitrous acid, radioactive inosine 5[image]-phosphate, chromatographically pure, can be separated in a similar manner. Radioactive ribose 5-phosphate is obtained by heating this adenosine 5[image]-phosphate in [image] HCL and can be separated by paper chromatography.Keywords
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