A multiplexed single-photon instrument for routine measurement of time-resolved fluorescence anisotropy

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Abstract
The authors report a new type of instrument, the Edinburgh Instruments Model 2997 differential fluorometer, for the measurement of the decay of fluorescence anisotropy. The instrument incorporates three multiplexed detection channels for simultaneous measurement of two polarised fluorescence decays and the excitation pulse, using time-correlated single-photon counting. This arrangement corrects automatically for temporal and intensity fluctuations of the spark source. A study of the probe 1,6-diphenyl-1,3,5-hexatriene (DPH) in a white oil reveals that the instrument can measure rotational correlation times with subnanosecond time resolution in a third of the time of conventional methods.