Chronic Helicobacter pylori Infection Does Not Significantly Alter the Microbiota of the Murine Stomach
- 1 February 2007
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 73 (3), 1010-1013
- https://doi.org/10.1128/aem.01675-06
Abstract
We examined the impact of Helicobacter pylori infection on the murine gastric microbiota by culture and terminal-restriction fragment length polymorphism and found that neither acute nor chronic H. pylori infection substantially affected the gastric microbial composition. Interestingly, the total H. pylori burden detected by real-time PCR was significantly higher than that revealed by viable counts, suggesting that the antigenic load sustaining H. pylori -induced gastritis could be considerably higher than previously believed.Keywords
This publication has 12 references indexed in Scilit:
- CD4+CD25+Regulatory T Cells Modulate the T-Cell and Antibody Responses inHelicobacter-Infected BALB/c MiceInfection and Immunity, 2006
- Vaccination preventsHelicobacter pylori-induced alterations of the gastric flora in miceFEMS Immunology & Medical Microbiology, 2006
- Profiling and Identification of Eubacteria in the Stomach of Mongolian Gerbils With and Without Helicobacter pylori InfectionHelicobacter, 2003
- Secretory Antibodies Do Not Affect the Composition of the Bacterial Microbiota in the Terminal Ileum of 10-Week-Old MiceApplied and Environmental Microbiology, 2003
- Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers setMicrobiology, 2002
- Post-immunisation gastritis and Helicobacter infection in the mouse: a long term studyGut, 2001
- SS1 Helicobacter pylori disrupts the paracellular barrier of the gastric mucosa and leads to neutrophilic gastritis in miceVirchows Archiv, 2001
- Profiling of bacterial flora in gastric biopsies from patients with Helicobacter pylori-associated gastritis and histologically normal control individuals by temperature gradient gel electrophoresis and 16S rDNA sequence analysisJournal of Medical Microbiology, 2000
- Omeprazole, Helicobacter pylori status, and alterations in the intragastric milieu facilitating bacterial N-nitrosationGastroenterology, 2000
- A standardized mouse model of Helicobacter pylori infection: Introducing the Sydney strainGastroenterology, 1997