Induction of prostaglandin E release from macrophages by colchicine.
- 1 January 1980
- journal article
- research article
- Published by The American Association of Immunologists in The Journal of Immunology
- Vol. 124 (1), 376-380
- https://doi.org/10.4049/jimmunol.124.1.376
Abstract
Rat peritoneal macrophages released high amounts of prostaglandin E (PGE) when treated in vitro with 10(-7) to 10(-4) M colchicine. PGE production occurred after a lag period of 4 hr and proceeded at a constant rate for more than 24 hr. Lymphocytes could not be stimulated to PGE release by colchicine. Disaggregation of microtubules appeared to be an essential event, since lumicolchicine was inactive and addition of heavy water (D2O) abolished colchicine-induced PGE formation. Cytochalasin B (5 microgram/ ml) did not interfere with PGE production by colchicine during the initial 12 hr, but thereafter it gave rise to an activity capable of degrading or converting newly synthesized PGE. Although details of the mechanisms by which colchicine in association with disrupted microtubules may induce PGE release remain unclear, these observations suggest that components of the cytoskeleton may efficiently influence the biosynthesis of prostaglandins.This publication has 1 reference indexed in Scilit:
- The Effects of Phagocytosis, Dextran Sulfate, and Cell Damage on PGE1 Sensitivity and PGE1 Production of MacrophagesThe Journal of Immunology, 1978