The Effect of Reduced Hydraulic Conductance on Stomatal Conductance and Xylem Cavitation

Abstract

The vulnerability of xylem conduits to cavitation theoretically determines the maximum flow rate of water through plants, and hence maximum transpiration (E), stomatal conductance (g_s), and leaf area (A₁. Field-grown Betula occidentalis with a favourable water supply exhibit midday xylem pressures (ψ_px) approaching the cavitation-inducing range (–1·42 to – 2·2 MPa). We studied the ability of the stomata to prevent cavitation-inducing pressures when whole-plant hydraulic conductance per leaf area (k₁ was reduced by making overlapping transverse cuts in the main stem. Controls were intact, or had only the phloem cut in the same pattern. Reducing k₁ caused two responses: (1) variable g_s or E with ψ_px falling into the cavitation range causing up to 98% embolism and 100% leaf death, (2) decreased g_s and E with ψ_px remaining above the cavitation point and no leaf death or induction of cavitation. Shoots avoiding cavitation either produced new xylem and returned to control values of k₁g_s and E (experiments in June and July), or they showed continued decline in g, and E associated with increasing ψ_px and eventual premature senescence of leaves (experiments in August). Whether embolism occurred after reducing k₁ probably depended on the response time of stomata, and the proximity of ψ_px to the cavitation range when the xylem was cut. Stomata probably responded indirectly to reduced k via small changes in leaf ψ root signalling was unlikely because of the constant rooting environment.