The control of immune response in vitro by Ca2+. II. The Ca2+-dependent period during mitogenic stimulation.
- 1 January 1975
- journal article
- Vol. 28 (1), 121-5
Abstract
The Ca2+ requirement for stimulation of DNA synthesis in vitro by phytohaemagglutinin (PHA) and by lipopolysaccharide (LPS) was investigated using mouse spleen cells. During mitogenic stimulation two periods could be distinguished: a Ca2+-independent period which was followed by a Ca2+-dependent period. The results suggest that Ca2+ is required for a step preceeding DNA synthesis but not for the early initial phase of transformation.This publication has 6 references indexed in Scilit:
- Control of the immune response in vitro by calcium ions. I. The antagonistic action of calcium ions on cell proliferation and on cell differentiation.1974
- Stimulation of DNA synthesis in mouse lymphoid cells by polyanions in vitro. I. Target cells and possible mode of actionEuropean Journal of Immunology, 1973
- Requirement for calcium ions in lymphocyte transformation stimulated by phytohemagglutininJournal of Cellular Physiology, 1972
- Guanosine 3′:5′-Cyclic Monophosphate: A Possible Intracellular Mediator of Mitogenic Influences in LymphocytesProceedings of the National Academy of Sciences, 1972
- Interaction of lymphocytes and phytohaemagglutinin: Inhibition by chelating agents*1Experimental Cell Research, 1971
- Metal Cation Requirements for Phytohemagglutinin-Induced Transformation of Human Peripheral Blood LymphocytesThe Journal of Immunology, 1970