Phenotypic and cytogenetic characterization of a cell line derived from primary prostatic carcinoma

Abstract

A new cell line, PPC‐1, has been established from a specimen obtained from a patient with a poorly differentiated adenocarcinoma of the prostate. This is the first line of its type derived from a primary prostatic tumor site. PPC‐1 cells have become immortalized in culture, exhibit transformation parameters including relaxed growth factor requirements and anchorage‐independent growth, and are highly tumorigenic in nude mice. Cytogenetic studies by G‐banding revealed a grossly abnormal karyotype, with a modal chromosome number of 84, multiple marker chromosomes including both homogeneously staining regions and double minutes and clonal loss of chromosomes 3, 5, 10, 15 and Y.