Adsorptive and electrochemical behaviors of estradiol valerate at a mercury electrode

Abstract

It was found that estradiol valerate could be adsorbed at a mercury electrode under open circuit. The adsorptive and electrochemical behaviors of estradiol valerate on a static mercury electrode were investigated by cyclic voltammetry, linear scan voltammetry and chronocoulometry. Based on this, a sensitive and selective adsorptive stripping square-wave voltammetric method was developed for the determination of estradiol valerate based on the optimization of solution conditions and electrochemical parameters. It was found that in a Britton–Robinson buffer solution containing 18% alcohol (pH 9.5), estradiol valerate gave a sensitive reductive peak at potential –1.29 V (vs. SCE) and the peak current was linear with the concentration of estradiol valerate in the range 2.0 × 10^–8–2.5 × 10^–6 mol L^–1. The detection limit was 1.1 × 10^–8 mol L^–1. The interference of some common steroid estrogens was examined and it was found that they did not interfere in the determination of estradiol valerate in the present system.