Cloning and DNA sequence analysis of the glucose oxidase gene from Aspergillus niger NRRL‐3
- 11 September 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 255 (1), 63-66
- https://doi.org/10.1016/0014-5793(89)81061-0
Abstract
A cDNA library from Aspergillus niger strain NRRL-3 enriched in sequences encoding the glucose oxidase was constructed. An 800 bp cDNA clone isolated from this library was used to screen 12 000 recombinant phages from an EMBL3 genomic library. A 15 kbp DNA segment isolated from this library contained the 1815 bp structural gene for glucose oxidase as well as a short 5′- and a longer 3′-noncoding region. The deduced protein sequence was verified by partial peptide sequencing.Keywords
This publication has 12 references indexed in Scilit:
- Three α‐amylase genes of Aspergillus oryzae exhibit identical intron‐exon organizationMolecular Microbiology, 1989
- Formation and location of glucose oxidase in citric acid producing mycelia of Aspergillus nigerApplied Microbiology and Biotechnology, 1985
- Transformation of Aspergillus nidulans by using a trpC plasmid.Proceedings of the National Academy of Sciences, 1984
- Lambda replacement vectors carrying polylinker sequencesJournal of Molecular Biology, 1983
- Evidence for an essential histidine residue in glucose dehydrogenase from Bacillus megaterium and sequence analysis of the peptides labeled with bromoacetyl pyridineEuropean Journal of Biochemistry, 1983
- Multiple forms of glucose oxidase with different carbohydrate compositionsBiochimica et Biophysica Acta (BBA) - Enzymology, 1981
- Isolation of biologically active ribonucleic acid from sources enriched in ribonucleaseBiochemistry, 1979
- Subunit structure of glucose oxidase from Aspergillus nigerBiochemistry, 1972
- A glycoprotein structure for glucose oxidase from Aspergillus nigerArchives of Biochemistry and Biophysics, 1965