Biochemical Analysis of Vagotomized Stomach Wall in Rats

Abstract

The authors carried out a biochemical analysis of vagotomized rat stomach wall 2–3 weeks after a bilateral vagotomy. The biochemical extraction was performed by the Schneider-Schmidt-Thannhauser method. The phosphate fractions were measured colorimetrically according to Briggs. The nucleic acids were measured on the basis of phosphate and sugar components according to Seibert and Brown. The following compounds were determined: acid-soluble organic and inorganic phosphates, phospholipidphosphate, ribonucleic acidic phosphate, deoxyribonucleic acidic phosphate, phosphoproteid inorganic phosphate, ribonucleic acid and deoxyribonucleic acid from the whole, the glandular and the membranaceous stomach. These were compared with the some constituents of the normal rat stomach. After vagotomy a decrease was observed in acid-soluble inorganic phosphate, in organic acid-soluble phosphate, in phosphoprotein inorganic phosphate of the glandular, membranaceous and the total stomach. The phospholipid phosphate did not decrease. There was no change in the content of ribonucleic acid in the membranaceous stomach, whereas the content of deoxyribonucleic acid showed an increase.