Induction of Cyclic AMP Phosphodiesterase by Disaggregation of the Multicellular Complexes of Dictyostelium discoideum
- 1 January 1979
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 93 (2), 221-227
- https://doi.org/10.1111/j.1432-1033.1979.tb12814.x
Abstract
When the multicellular complexes (slugs) of D. discoideum were disaggregated, the activity of phosphodiesterase [EC-3.1.4.1] for cyclic(c)AMP increased. The increase occurred first with the enzyme in the particulate fraction of the cell. Cytoplasmic and extracellular phosphodiesterase and the macromolecular inhibitor for this enzyme also increased later. The increase in these activities was blocked by inhibitors of protein and RNA syntheses. This indicates that the increase is not due to the activation of the enzyme, but due to its synthesis de novo. The synthesis is induced at the transcriptional level by the loss of cell contact. In the case of cellular phosphodiesterase, inhibitors of protein synthesis (cycloheximide and emetine) not only blocked the increase, but also caused a decrease in activity. The mechanism of this decrease was further investigated.This publication has 18 references indexed in Scilit:
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