A complex of rab3A, SNAP‐25, VAMP/synaptobrevin‐2 and syntaxins in brain presynaptic terminals
- 13 September 1993
- journal article
- Published by Wiley in FEBS Letters
- Vol. 330 (2), 236-240
- https://doi.org/10.1016/0014-5793(93)80281-x
Abstract
Two monoclonal antibodies (SPM-1 and SPM-2) immunoprecipitate brain N-type calcium channels. On immunoaffinity chromatography of digitonin extracts of bovine brain membranes on SPM-1- and SPM-2-Sepharose, proteins of 36 (syntaxins A and B), 28 and 19 kDa are specifically retained by both columns. Here we show that the 19 and 28 kDa bands contain VAMP/synaptobrevin-2, and rab3A/smg25A and SNAP-25, respectively. Since SPM-1 and SPM-2 recognize only syntaxins and the 28 kDa band (rab3A/smg25A and SNAP-25), respectively, the results indicate that all these proteins form a complex. Our results suggest tight linkage between the components involved in neurotransmitter release.Keywords
This publication has 26 references indexed in Scilit:
- SNAP family of NSF attachment proteins includes a brain-specific isoformNature, 1993
- SNAP receptors implicated in vesicle targeting and fusionNature, 1993
- Tetanus and botulinum-B neurotoxins block neurotransmitter release by proteolytic cleavage of synaptobrevinNature, 1992
- Syntaxin: A Synaptic Protein Implicated in Docking of Synaptic Vesicles at Presynaptic Active ZonesScience, 1992
- Monoclonal antibodies immunoprecipitating ω-conotoxin-sensitive calcium channel molecules recognize two novel proteins localized in the nervous systemBiochemical and Biophysical Research Communications, 1991
- Association of Rab3A with synaptic vesicles at late stages of the secretory pathway.The Journal of cell biology, 1991
- A small GTP-binding protein dissociates from synaptic vesicles during exocytosisNature, 1991
- The identification of a novel synaptosomal-associated protein, SNAP-25, differentially expressed by neuronal subpopulations.The Journal of cell biology, 1989
- A fusion protein required for vesicle-mediated transport in both mammalian cells and yeastNature, 1989
- A synaptic vesicle membrane protein is conserved from mammals to DrosophilaNeuron, 1989