Determination of the translation start site of the large subunit of ribulose-1,5-bisphosphate carboxylase from maize
- 1 January 1984
- journal article
- research article
- Published by Springer Nature in Plant Molecular Biology
- Vol. 3 (6), 403-406
- https://doi.org/10.1007/bf00033388
Abstract
Sequence studies of the gene for the large subunit of ribulose-1,5-bisphosphate carboxylase from maize indicate the presence of two translation start sites, 18 bp apart. Each site is preceded by a suitable ribosome binding region. By using a simplified E. coli-based in vitro system which measures fromation of the first dipeptide of the gene product, we have determined that only the second methionine initiates translation.Keywords
This publication has 24 references indexed in Scilit:
- Synthesis of the large subunit of ribulose-1,5-bisphosphate carboxylase in anin vitro partially definedE. coli systemPlant Molecular Biology, 1983
- Quantitative isolation of DNA restriction fragments from low-melting agarose by Elutip-d affinity chromatographyAnalytical Biochemistry, 1983
- The Nucleotide Sequence for the Large Subunit of Ribulose 1,5-Bisphosphate Carboxylase from a Unicellular Cyanobacterium,SynechococcusPCC6301DNA, 1983
- Sequence of the chloroplast DNA region of Chlamydomonas reinhardii containing the gene of the large subunit of ribulose bisphosphate carboxylase and parts of its flanking genesJournal of Molecular Biology, 1982
- The nucleotide sequence of the tobacco chloroplast gene for the large subunit of ribulose-l,5-bisphosphate carboxylase/oxygenaseGene, 1982
- In vitro expression of Escherichia coli ribosomal protein L10 gene: Tripeptide synthesis as a measure of functional mRNAArchives of Biochemistry and Biophysics, 1982
- Photochrome-mediated regulation of messenger RNAs for the small subunit of ribulose 1, 5-bisphosphate carboxylase and the light-harvesting chlorophyll a/b-protein in Lemna gibbaPlant Molecular Biology, 1981
- Chloroplast gene sequence for the large subunit of ribulose bisphosphatecarboxylase of maizeNature, 1980
- Protein synthesis in chloroplasts I. Light-driven synthesis of the large subunit of Fraction I protein by isolated pea chloroplastsBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Studies on fraction I protein IV. Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains the code for a chloroplast proteinBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1972