STUDIES ON A PROTAMINE (GALLINE) FROM FOWL SPERM .3. TOTAL AMINO-ACID SEQUENCE OF INTACT GALLINE MOLECULE

Abstract

Of the 8 fractions (G-I-G-VIII) obtained from galline, a protamine from rooster [Gallus domesticus] sperm nuclei, the complete amino acid sequences of 2 fractions G-I and G-V were already reported. Other fractions of galline purified repeatedly on the column of Bio-Gel CM[carboxymethyl]-30 were submitted to structural analyses. The complete amino acid sequences of 3 (G-II, G-III and G-VII) of the fractions and the partial chemical structures of 2 (G-VI and G-VIII) of the fractions were established from the results of analyses of the tryptic and chymotryptic peptides together with those of end groups and terminal sequences. Remarkable similarities were found in the sequences of these fractions. The intact molecule is composed of apparently only 1 molecular species (G-VIII) and fractions (G-I-G-VII) were derived from G-VIII by the action of an inherent protease in the preparation process. The total amino acid sequence of the intact galline is as follows: .**GRAPHIC**. Arg-Tyr-Arg-Ser-Arg-Gly-Arg-Ser-Arg-Ser-Arg-Arg-Thr-Arg-Arg-Arg-Arg-Ser-Pro-Arg-Ser-Gly-Arg-Arg-Arg-Ser-Pro- Arg-Arg-Arg-Arg-Ser-Arg-Arg-Arg-Arg-Arg-Tyr-Gly-Ser-Ala-Arg-Arg-Ser-Arg-Arg-Ser-Gly-Gly-Val-Arg-Arg-Arg-Arg-Tyr-Gly-Ser-Arg-Arg-Arg-Arg-Arg-Arg .**GRAPHIC**.