Determination of Ofloxacin, A New Oxazine Derivative in Human Serum, Urine, and Bile by High-Performance Liquid Chromatography

Abstract
A simple and precise high-performance liquid procedure has been developed for the determination of Ofloxacin (DL-8280), a new oxazine derivative in human serum, urine and bile using Norfloxacin as internal standard. The work-up procedure involves a chemical extraction step followed by isocratic chromatography on a anion-exchange analytical column, with ultraviolet detection. The run time for the assay was 11.5 minutes.

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