Self-Transfer and Genetic Recombination Mediated by P, the Sex Factor of Vibrio cholerae

Abstract

Vibrio cholerae cells, infected with the sex factor P, produce discrete, plaque-like clearings when plated on lawns of P⁻ cells. We investigated the nature of these clearings and conclude that they are probably sites of active mating. We developed a quantitative assay for P⁺ cells and used it to study the kinetics of sex factor spread in broth cultures. Both established and newly infected donor populations were efficient sex factor donors, indicating that P is not self-repressed. We also investigated the kinetics of recombinant formation in broth matings. In 1-hr matings, we routinely found recombination frequencies of 10⁻⁶ per donor cell. Kinetic studies of recombinant formation showed that the markers tested all appeared at early times. Thus P, the V. cholerae sex factor, seems to resemble F in its transfer properties.