THE INCORPORATION OF LEUCINE-C 14 INTO PROTEIN BY A CELL-FREE PREPARATION FROM MAIZE KERNELS
- 1 April 1960
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 46 (4), 484-488
- https://doi.org/10.1073/pnas.46.4.484
Abstract
A system from developing maize endosperm consisting of washed particles plus a pH 5.2 fraction from the supernatant that actively incorporates leucine-Cl4 into protein is described. The system requires a source of energy and GTP. The incorporation is inhibited by treatment with ribonuclease or chlor-amphenicol. When operating optimally, the incorporation efficiency is 1 to 2% of the added isotope, making this system comparable to the mammalian systems.This publication has 5 references indexed in Scilit:
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