ISOLATION OF HUMAN EOSINOPHILS AND THEIR LYMPHOCYTE-LIKE ROSETTING PROPERTIES
- 1 January 1976
- journal article
- research article
- Vol. 31 (3), 337-341
Abstract
A method for the isolation of large numbers of almost pure human eosinophils from peripheral blood using hypaque-ficoll density gradients, gelatin sedimentation and carbonyl iron is described. Eosinophils were obtained from patients with parasitic infections, allergic reactions, undiagnosed eosinophilias and normal volunteers. Erythrocyte (E) [sheep] and E-antibody-complement (EAC) rosettes were formed by a number of eosinophils.This publication has 6 references indexed in Scilit:
- Role of the eosinophil in the allergic reactions. I. EDI-an eosinophil-derived inhibitor of histamine release.1975
- SURFACE MARKERS ON HUMAN T AND B LYMPHOCYTESThe Journal of Experimental Medicine, 1972
- Eosinophil cell separation from human peripheral blood.1970
- AN EXTENSION OF THE51Cr-RELEASE ASSAY FOR THE ESTIMATION OF MOUSE CYTOTOXINSTransplantation, 1968
- Isolation of mononuclear cells and granulocytes from human blood. Isolation of monuclear cells by one centrifugation, and of granulocytes by combining centrifugation and sedimentation at 1 g.1968