Biochemically Marked Lymphocytoid Lines: Establishment of Lesch-Nyhan Cells
- 2 October 1970
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 170 (3953), 89-90
- https://doi.org/10.1126/science.170.3953.89
Abstract
Two lymphocytoid cell lines have been established from a patient with the Lesch-Nyhan syndrome. The cells are deficient in hypoxanthine-guanine phosphoribosyltransferase, as demonstrated by their failure to incorporate [H3]hypoxanthine and by their inability to grow in medium in which they were nutritionally dependent upon exogenous hypoxanthine. This represents the first establishment of presumptively permanent human lymphocytoid cell lines that are deficient in a specific enzyme.Keywords
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