Purification and amino acid sequence of the ovulation neurohormone of Lymnaea stagnalis
- 1 November 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (22), 7767-7771
- https://doi.org/10.1073/pnas.82.22.7767
Abstract
The neurosecretory caudodorsal cells of the freshwater pulmonate snail Lymnaea stagnalis produce an ovulation hormone [caudodorsal cell hormone (CDCH)] that is stored and released at the periphery of the intercerebral commissure. In the present study, CDCH has been purified and sequenced by micromethods. CDCH has been isolated, starting with a hydrochloric acid extract of commissures. By chromatofocusing, by high-performance, gel-permeation chromatography, and by reversed-phase, high-performance liquid chromatography. This procedure resulted in a 1690-fold purification and a 66% recovery. The data of the sequence analysis of CDCH are in agreement with the amino acid composition and reveal the following sequence of 36 amino acids: H-Leu-Ser-Ile-Thr-Asn-Asp-Leu-Arg-Ala-Ile-Ala-Asp-Ser-Tyr-Leu-Tyr-Asp-Gln-His-Trp-Leu-Arg-Glu-Arg-Gln-Glu-Glu-Asn-Leu-Arg-Arg-Arg-Phe-Leu-Glu-Leu-OH. Enzyme data indinate that the COOH end of the hormone is amidated. CDCH has a calculated isoelectric point of 9.0 and a calculated M4 of 4529. CDCH shares a 44% homology with the sequence of the egg-laying hormone of the marine opisthobranch mollusc Aplysia californica.Keywords
This publication has 20 references indexed in Scilit:
- An identified neuron modulating the activity of the ovulation hormone producing Caudo-Dorsal cells of the pond snailLymnaea stagnalisJournal of Neurobiology, 1984
- Isolation of neurosecretory granules from the neurohaemal areas of peptidergic systems of Lymnaea stagnalis, with special reference to the ovulation hormone-producing caudodorsal cellsGeneral and Comparative Endocrinology, 1984
- Synchronous maturation of the female reproductive system and of the ovulation hormone-producing system in Lymnaea stagnalisGeneral and Comparative Endocrinology, 1983
- Multiple release of peptides by electrically active neurosecretory caudo-dorsal cells of Lymnaea stagnalisNeuroscience Letters, 1983
- Partial purification and characterization of the ovulation hormone of the freshwater pulmonate snail Lymnaea stagnalisGeneral and Comparative Endocrinology, 1983
- A rapid in vivo bioassay of the ovulation hormone of Lymnaea StagnalisGeneral and Comparative Endocrinology, 1983
- Effect of the photoperiod on the time schedule of egg mass production in Lymnaea stagnalis, as induced by ovulation hormone injectionsGeneral and Comparative Endocrinology, 1983
- A single gene encodes multiple neuropeptides mediating a stereotyped behaviorCell, 1983
- States of excitability in ovulation hormone producing neuroendocrine cells ofLymnaea stagnalis (gastropoda) and their relation to the egg-laying cycleJournal of Neurobiology, 1980
- The control of ovulation in the hermaphroditic freshwater snail Lymnaea stagnalis by the neurohormone of the caudodorsal cellsGeneral and Comparative Endocrinology, 1976