Isolation of several cDNAs encoding yeast peroxisomal enzymes

Abstract

Several candidate clones carrying partial cDNAs for yeast peroxisomal enzymes, such as catalase, carnitine acetyltransferase, isocitrate lyase, malate synthase and acyl‐CoA oxidase, were efficiently isolated at a single plating from a phage λgt11 recombinant cDNA library prepared with poly(A)‐rich RNA from an n‐alkane‐grown yeast, Candida tropicalis, with a mixture of antibodies against the respective purified enzymes. Among them, one candidate clone carrying partial cDNA for catalase was subcloned and subjected to nucleotide sequence analysis. We succeeded in determining that the amino acid sequence deduced from the nucleotide analysis included the sequences derived from the two peptide fragments obtained from the purified enzyme.