Radioimmunoassay of Plasma 16a-Hydroxyprogesterone
- 1 August 1973
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 6 (8), 675-683
- https://doi.org/10.1080/00032717308058720
Abstract
This report describes a radioimmunoassay method for the measurement of unconjugated 16a-hydroxyprogesterone (16a-P) in plasma. Utilizing an antiserum against 16a-P combined with a one step celite microcolumn chromatographic system, we have measured this steroid accurately in small a liquors of plasma from both male and female subjects. The mean value ± S.D. for nine adult male subjects was 0.69 ± 0.16 ng/ml. Plasma 16a-P was significantly higher (p <0.01) during the luteal (1.20 ± 0.24 ng/ml) than during the follicular phase (0.57 ± 0.18 ng/ml).Keywords
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