EXPERIMENTAL VERIFICATION OF THE ASSUMPTION UNDERLYING THE COLOUR CORRECTION EQUATION OF ALLEN

Abstract

Estrone methyl ether, estradiol-17 beta-3-mono-methyl ether, estriol-3-methylether, and pregnane-3 alpha, 20 alpha-diol diacetate obtained from urinary extracts and progestorone obtained from placental extracts were separated by countercurrent distribution from interfering substances. When the interfering substances were determined by the same colorimetric reaction as the steroid the absorption curve of interfering substances was linear in the region of the absorption maximum of the steroid measured and a close agreement existed between theoretical and experimental countercurrent values for each steroid when the experimental values were corrected according to the formula of Allen.