Reduced Leucocyte Alkaline Phosphatase Activity and Decreased NBT Reduction Test in Induced Iron Deficiency Anaemia in Rabbits

Abstract
Fe deficiency anemia was induced in rabbits by repeated bleeding. The leukocyte alkaline phosphatase (LAP) of 26 .+-. 28 units [U] was significantly reduced compared with control values of 233 .+-. 36 U (P < 0.001). Leukocyte NBT reduction was also diminished, in Hanks solution (P < 0.01) and in autologous serum (P < 0.001). After administration of Fe these values returned to normal. Reduced LAP may reflect a deficiency of Fe-dependent constituents necessary for integrity of normal granulocyte metabolism.