Rapid modification of the morphology of cell contact sites during the aggregation of limpet haemocytes

Abstract

Haemocytes suspended in the blood of the limpet Patella vulgata rapidly form microspikes and aggregate in response to withdrawal from the haemocoel. It has previously been suggested that the contacts between colliding cells in shaken suspension are stabilized by the rapid spreading of participant cells over each other’s surfaces by a microspike-dependent process. In this communication we report that an electron-microscopic study of the contacts formed between cells in the early stages of aggregation suggests that intercellular contact elicits a rapid response from the organelles concerned with cell motility. This response leads to the rapid progression of each initial collision through 3 major categories of contact morphology, namely cell body/lamella, lamella/lamella and cell body/cell body. There is a concomitant increase in the area of mutually contacting surface between participating cells. On the basis of these results, a model is constructed for the participation of sub-surface components in the process of cellular aggregation. The sequence of development of contact found between aggregating cells is envisaged to be a consequence of cellular locomotory activities resembling those by which these cells spread on to a solid substrate. Included in this process is an element of intercellular recognition. It is suggested that the involvement of motile elements in cell aggregation is a general feature of aggregating cells. This would permit a re-interpretation of aggregation experiments which show specificity of cell adhesion, for total adhesive interactions between cells would depend not only on the adhesiveness per unit area of contacting cell surface but also on the extent or rate of formation of flat contact areas. The latter element could be regulated by a recognition system between contacting cells independently of the force per unit area which holds the 2 surfaces together.