ANTIGENS OF ASPERGILLUS-FUMIGATUS .1. PURIFICATION OF A CYTOPLASMIC ANTIGEN REACTIVE WITH SERA OF PATIENTS WITH ASPERGILLUS-RELATED DISEASE
- 1 January 1981
- journal article
- research article
- Vol. 45 (3), 662-671
Abstract
An antigen is purified from the mycelial cell sap of A. fumigatus (strain 507). The same component appears to be present in the extracellular phase (culture filtrate) in a partially degraded form. The cell sap protein has a structure composed of 4 polypeptides of 45,000 daltons linked through disulfide bonds. The isoelectric point (5.2-5.6) and carbohydrate content (12.5% neutral hexose) indicate that this proteins is an acidic glycoprotein. It shows reactions with 75% of sera from patients with aspergilloma and allergic bronchopulmonary aspergillosis and is not reactive with sera from normal individuals or patients with other fungal diseases. It also appears to be a component of other A. fumigatus strains.This publication has 21 references indexed in Scilit:
- The Detection of Circulating Antibodies Against Antigens from Three Strains of Aspergillus fumigatus*Mycoses, 2009
- Immunologic Lung DiseaseNew England Journal of Medicine, 1979
- Evaluation of Methods to Detect Antibodies against Aspergillus fumigatusAmerican Journal of Clinical Pathology, 1978
- Variability of extracts used in immunoprecipitin testsJournal of Allergy and Clinical Immunology, 1977
- Studies in chronic allergic bronchopulmonary aspergillosis. 3. Immunological findings.Thorax, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- A high resolution PAS stain for polyacrylamide gel electrophoresisAnalytical Biochemistry, 1973
- Identification and quantitation of antigens and antibodies by means of quantitative immunoelectrophoresis. A survey of methodsJournal of Immunological Methods, 1972
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Pulmonary aspergillosis: Diagnostic and immunological significance of antigens and C‐substance in Aspergillus fumigatusThe Journal of Pathology and Bacteriology, 1964