Changes in the activity of acetyl-CoA carboxylase during rape-seed formation

Abstract

During the formation of rape seeds, lipid accumulated in the cotyledons from 16 days after pollination, rising to a plateau after 28 days. The accumulation of lipid was preceded by a marked rise in acetyl-CoA carboxylase activity, which declined rapidly, correlating with the decline in rate of lipid formation. Incubation of rape-seed extracts with avidin-agarose resulted in a decrease in acetyl-CoA carboxylase activity in the extract. Polyacrylamide-gel electrophoresis of polypeptides bound to avidin-agarose showed the presence of a polypeptide of MW 225,000. The intensity of this band increased during the period of increase of acetyl-CoA carboxylase activity in the seeds.